Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-vinyl sphinganine phosphate | 50% inhibition at 0.0024 mM | Rattus norvegicus | |
4-chloromercuribenzoate | alkylates a cysteine residue | Mammalia | |
Ca2+ | potent inhibitor | Mammalia | |
cyanide | inhibits | Mammalia | |
deoxypyridoxin phosphate | inhibits | Mammalia | |
iodoacetamide | alkylates a cysteine residue | Mammalia | |
N,N-dimethyldodecylamine N-oxide | 50% inhibition at 0.05 mM | Mammalia | |
N-ethylmaleimide | alkylates a cysteine residue | Mammalia | |
Semicarbazide | inhibits | Mammalia | |
sphinganine 1-phosphonate | competitive inhibitor | Rattus norvegicus | |
sphinganine phosphate | 2D,3L-isomer is a competitive inhibitor, but 2L,3L-isomer does not influence the enzyme | Tetrahymena pyriformis | |
Zn2+ | potent inhibitor | Mammalia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | 0.016 | sphinganine 1-phosphate | - |
Rattus norvegicus | |
0.11 | - |
sphinganine 1-phosphate | - |
Tetrahymena pyriformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | catalytic site faces the cytosol | Mammalia | 5783 | - |
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | 65000 | SDS-PAGE | Mus musculus |
61000 | 65000 | SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mammalia | acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mammalia | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Tetrahymena pyriformis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde | mechanism | Mammalia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
additional information | no activity in platelets | Mammalia | - |
Storage Stability | Organism |
---|---|
-80°C, activity is well preserved in tissues or cells | Mammalia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine | Mammalia | ? | - |
? | |
additional information | acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine | Mus musculus | ? | - |
? | |
additional information | acts on 1-phosphorylated derivatives of sphingoid bases, products are an aliphatic fatty aldehyde and phosphoethanolamine | Homo sapiens | ? | - |
? | |
sphinganine 1-phosphate | - |
Rattus norvegicus | phosphoethanolamine + palmitaldehyde | - |
? | |
sphinganine 1-phosphate | - |
Tetrahymena pyriformis | phosphoethanolamine + palmitaldehyde | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mammalia |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
sphinganine 1-phosphonate | - |
Rattus norvegicus | |
0.0097 | - |
sphinganine phosphate | 2D,3L-isomer | Tetrahymena pyriformis |